Dear colleagues,
I would like to invite you to the lecture by Matěj Šamaj "Evaluation of a 53BP1 Reporter Cell Line for DNA Damage Response and Screening Applications" The lecture will be on Wednesday June 17, 2026 at 9AM in the room 1.09 at IMTM.
Abstract
DNA double-strand breaks trigger the rapid recruitment of 53BP1 to sites of damage, resulting in the formation of distinct nuclear foci. Utilizing a U2OS-53BP1-EYFP reporter cell line, this system enables real-time visualization of these foci dynamics over time. In this work, DNA damage was induced by X-ray irradiation and combined with reference DNA damage response (DDR) inhibitors to characterize changes in 53BP1 foci formation and resolution. The results demonstrate the utility of this system for investigating DNA repair mechanisms and support its future use in compound screening applications.